Use of Modified PCR Ribotyping for Direct Detection of Clostridium difficile Ribotypes in Stool Samples

July 30, 2011 at 5:32 pm Leave a comment

J Clin Microbiol 1 Aug 2011 V.49 N.8 P.3024-3025

Notes

Sandra Janei1, Iztok trumbelj2 and Maja Rupnik1,3,4*

Institute of Public Health Maribor, Prvomajska 1, Maribor, Slovenia,1

Institute of Public Health Murska Sobota, Ulica Arhitekta Novaka 2b, Murska Sobota, Slovenia,2

University of Maribor, Faculty of Medicine, Slomskov trg 15, Maribor, Slovenia,3

Centre of Excellence Cipkebip, Jamova 39, Ljubljana, Slovenia4

PCR ribotyping was modified to allow direct detection of Clostridium difficile from stool samples. Direct PCR ribotyping was possible in 86 out of99 C. difficile-positive stool samples, and in 84 cases (84.8%), the ribotype determined directly from the stool sample was identical to the ribotype of the strain isolated from the same stool sample.

abstract

http://jcm.asm.org/cgi/content/abstract/49/8/3024

PDF

http://jcm.asm.org/cgi/reprint/49/8/3024

Entry filed under: Bacterias, Biología Molecular, Infecciones gastrointestinales, Metodos diagnosticos.

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