Identification and characterization of non-tuberculous mycobacteria isolated from tuberculosis suspects in Southern-central China.
PLoS One. 2014 Dec 2;9(12):e114353.
Yu XL1, Lu L1, Chen GZ1, Liu ZG1, Lei H1, Song YZ2, Zhang SL3.
1School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
2Shanghai public health clinical center, Shanghai, China.
3Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
The incidence of non-tuberculous mycobacteria (NTM)-related death has increased globally recently.
To obtain information of the species and characterization of pathogens involved in NTM pulmonary infection in Southern-central China, we identified 160 non-tuberculous infection cases from 3995 acid-fast bacilli (AFB)-positive tuberculous suspects.
We then randomly selected 101 non-tuberculous patients, isolated bacteria from their sputa and genotyped the pathogens using the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences.
M. intracellulare (32.67%, 33/101), M. abscessus (32.67%, 33/101) and M. fortuitum (7.92%, 8/101) are identified in these isolates. Surprisingly, non-mycobacteria including Gordonia (8.91%, 9/101), Nocardia (5.94%, 6/101) and Tsukamurella (0.99%, 1/101) are also discovered, and the case of Tsukamurella pulmonis infection is first discovered in Southern-central China.
Moreover, species of M. mucogenicum group, M. chubuense, M. kansasii, M. gastri, M. avium, M. porcinum and M. smegmatis are identified.
In addition, nine immune compromised cases (8.91%, 9/101), including type two diabetes mellitus and HIV/AIDS are found to be infected with non-tuberculous bacteria.
This study revealed the distribution and characteristics of non-tuberculous AFB pathogen infection occurred in Southern-central China, and suggested that physicians should be alert of the emerging of NTM and non-mycobacteria infection in AFB positive cases and take caution when choosing chemotherapy for tuberculosis-like pulmonary infections.
Generally, this study may help with the development of new strategy for the diagnosis and treatment of mycobacterial infection.