Sensitivities, specificities, and predictive values of microbiological culture techniques for the diagnosis of prosthetic joint infection.

July 25, 2015 at 10:50 am

Biomed Res Int. 2014;2014:180416.

Jordan RW1, Smith NA2, Saithna A1, Sprowson AP2, Foguet P1.

Author information

1University Hospital Coventry & Warwickshire, Clifford Bridge Road, Coventry CV2 2DX, UK.

2University of Warwick, Coventry CV4 7AL, UK.

Abstract

BACKGROUND:

Identifying the microorganism in a prosthetic joint infection is the key to appropriately targeting antimicrobial treatment. Despite the availability of various techniques, no single test is considered the definitive gold standard.

AIM:

Our aim was to determine the sensitivity, specificity, and positive/negative predictive values for a variety of culture techniques.

METHODS:

We performed a retrospective case series of 219 patients undergoing revision surgery of their hip or knee replacement between May 2004 and February 2013. The patients were classified as either infected or noninfected according to criteria set out by the Musculoskeletal Infection Society. The number and type of samples taken intraoperatively varied between cases but included tissue samples and fluid sent in either blood culture vials or sterile containers.

RESULTS:

The highest sensitivity was found with blood culture vials (0.85) compared to fluid in sterile containers (0.26) and tissues samples (0.32). Blood culture vials also had a better specificity and positive and negative predictive values profile.

CONCLUSION:

We conclude that, of the techniques studied, fluid in blood culture vials had the best profile for the correct identification of microorganisms and advocate its use.

PDF

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055545/pdf/BMRI2014-180416.pdf

Entry filed under: Antimicrobianos, Bacterias, Biología Molecular, Health Care-Associated Infections, Infecciones nosocomiales, Infecciones osteo-articulares-musculares, Infecciones relacionadas a prótesis, Metodos diagnosticos, Resistencia bacteriana, Sepsis. Tags: .

The value of synovial biopsy, joint aspiration and C-reactive protein in the diagnosis of late peri-prosthetic infection of total knee replacements. Efficacy of a novel PCR- and microarray-based method in diagnosis of a prosthetic joint infection.


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