A novel acute HIV infection staging system based on 4th generation immunoassay

August 14, 2015 at 8:32 am

Retrovirology 2013, 10:56

SHORT REPORT

Jintanat Ananworanich1,2,3,4*, James LK Fletcher1,2, Suteeraporn Pinyakorn1,2,3, Frits van Griensven2,3, Claire Vandergeeten5, Alexandra Schuetz6, Tippawan Pankam2, Rapee Trichavaroj6, Siriwat Akapirat6, Nitiya Chomchey1,2, Praphan Phanuphak1,2,3,4, Nicolas Chomont5, Nelson L Michael7, Jerome H Kim1,7, Mark de Souza1,2 and on behalf of the RV254/SEARCH 010 Study Group

Background

Fourth generation (4thG) immunoassay (IA) is becoming the standard HIV screening method but was not available when the Fiebig acute HIV infection (AHI) staging system was proposed. Here we evaluated AHI staging based on a 4thG IA (4thG staging).

Findings

Screening for AHI was performed in real-time by pooled nucleic acid testing (NAT, n=48,828 samples) and sequential enzyme immunoassay (EIA, n=3,939 samples) identifying 63 subjects with non-reactive 2nd generation EIA (Fiebig stages I (n=25), II (n=7), III (n=29), IV (n=2)). The majority of samples tested (n=53) were subtype CRF_01AE (77%). NAT+ subjects were re-staged into three 4thG stages: stage 1 (n=20; 4th gen EIA-, 3rd gen EIA-), stage 2 (n=12; 4th gen EIA+, 3rd gen EIA-), stage 3 (n=31; 4th gen EIA+, 3rd gen EIA+, Western blot-/indeterminate). 4thG staging distinguishes groups of AHI subjects by time since presumed HIV exposure, pattern of CD8+ T, B and natural killer cell absolute numbers, and HIV RNA and DNA levels. This staging system further stratified Fiebig I subjects: 18 subjects in 4thG stage 1 had lower HIV RNA and DNA levels than 7 subjects in 4thG stage 2.

Conclusions

Using 4th generation IA as part of AHI staging distinguishes groups of patients by time since exposure to HIV, lymphocyte numbers and HIV viral burden. It identifies two groups of Fiebig stage I subjects who display different levels of HIV RNA and DNA, which may have implication for HIV cure. 4th generation IA should be incorporated into AHI staging systems.

PDF

http://www.retrovirology.com/content/pdf/1742-4690-10-56.pdf

Entry filed under: Biología Molecular, HIV/SIDA, HIV/SIDA Infeccion Aguda / Reciente, Metodos diagnosticos. Tags: .

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