KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil.

August 21, 2015 at 8:10 am

Braz J Microbiol. 2015 Jun 1;46(2):501-4.

Biberg CA1, Rodrigues AC2, do Carmo SF2, Chaves CE3, Gales AC4, Chang MR1.

1Universidade Federal de Mato Grosso do Sul, Laboratório de Pesquisas Microbiológicas, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil, Laboratório de Pesquisas Microbiológicas, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.

2Hospital Regional de Mato Grosso do Sul, Campo Grande, MS, Brasil, Laboratório de Microbiologia do Hospital Regional Rosa Pedrossian de Mato Grosso do Sul, Campo Grande, MS, Brazil.

3Hospital Regional de Mato Grosso do Sul, Campo Grande, MS, Brasil, Comissão de Controle de Infecção Hospitalar, Hospital Regional Rosa Pedrossian de Mato Grosso do Sul, Campo Grande, MS, Brazil.

4Universidade Federal de São Paulo, Laboratório Alerta, Divisão de Doenças Infecciosas, Universidade Federal de São Paulo, São Paulo, SP, Brasil, Laboratório Alerta, Divisão de Doenças Infecciosas, Universidade Federal de São Paulo, São Paulo, SP, Brazil.

Abstract

The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals.

In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized.

Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry.

The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines.

Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing.

Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the bla KPC-2 gene.

Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.

PDF

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507542/pdf/1517-8382-bjm-46-2-501.pdf

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Biología Molecular, Epidemiología, Metodos diagnosticos, Resistencia bacteriana, Sepsis, Update. Tags: .

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