Archive for February, 2016

Neuroinvasive West Nile Infection Elicits Elevated and Atypically Polarized T Cell Responses That Promote a Pathogenic Outcome

Public Library of Science Pathogens February 21, 2016

Eddie A. James, Theresa J. Gates, Rebecca E. LaFond, Shinobu Yamamoto, Chester Ni, Duy Mai, Vivian H. Gersuk, Kimberly O’Brien, Quynh-Anh Nguyen, Brad Zeitner, Marion C. Lanteri, Philip J. Norris, Damien Chaussabel, Uma Malhotra, William W. Kwok

Eddie A. James, Theresa J. Gates, Rebecca E. LaFond, Shinobu Yamamoto, Chester Ni, Duy Mai, Vivian H. Gersuk, Kimberly O’Brien, Quynh-Anh Nguyen, Brad Zeitner, Damien Chaussabel, William W. Kwok

Benaroya Research Institute at Virginia Mason, Seattle, Washington, United States of America

Marion C. Lanteri, Philip J. Norris

Blood Systems Research Institute, San Francisco, California, United States of America

Philip J. Norris

Departments of Laboratory Medicine and Medicine, University of California, San Francisco, San Francisco, California, United States of America

Uma Malhotra

Virginia Mason Medical Center, Seattle, Washington, United States of America

Uma Malhotra, William W. Kwok

Department of Medicine, University of Washington, Seattle, Washington, United States of America

Most West Nile virus (WNV) infections are asymptomatic, but some lead to neuroinvasive disease with symptoms ranging from disorientation to paralysis and death. Evidence from animal models suggests that neuroinvasive infections may arise as a consequence of impaired immune protection. However, other data suggest that neurologic symptoms may arise as a consequence of immune mediated damage. We demonstrate that elevated immune responses are present in neuroinvasive disease by directly characterizing WNV-specific T cells in subjects with laboratory documented infections using human histocompatibility leukocyte antigen (HLA) class II tetramers. Subjects with neuroinvasive infections had higher overall numbers of WNV-specific T cells than those with asymptomatic infections. Independent of this, we also observed age related increases in WNV-specific T cell responses. Further analysis revealed that WNV-specific T cell responses included a population of atypically polarized CXCR3+CCR4+CCR6- T cells, whose presence was highly correlated with neuroinvasive disease. Moreover, a higher proportion of WNV-specific T cells in these subjects co-produced interferon-γ and interleukin 4 than those from asymptomatic subjects. More globally, subjects with neuroinvasive infections had reduced numbers of CD4+FoxP3+ Tregs that were CTLA4 positive and exhibited a distinct upregulated transcript profile that was absent in subjects with asymptomatic infections. Thus, subjects with neuroinvasive WNV infections exhibited elevated, dysregulated, and atypically polarized responses, suggesting that immune mediated damage may indeed contribute to pathogenic outcomes.

FULL TEXT

http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005375

PDF

http://www.plospathogens.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.ppat.1005375&representation=PDF

 

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February 28, 2016 at 12:36 pm

Surveillance of device-associated infection rates and mortality in 3 Greek intensive care units.

Am J Crit Care. 2013 May;22(3):e12-20.

Apostolopoulou E1, Raftopoulos V, Filntisis G, Kithreotis P, Stefanidis E, Galanis P, Veldekis D.

Author information

1Nursing Department, National and Kapodistrian University of Athens, Greece.

Abstract

BACKGROUND:

Several studies suggest that device-associated, health care-associated infections (DA-HAIs) affect the quality of care in intensive care units, increasing patients’ morbidity and mortality and the costs of patient care.

OBJECTIVES:

To assess the DA-HAIs rates, microbiological profile, antimicrobial resistance, and crude excess mortality in 3 intensive care units in Athens, Greece.

METHODS:

A prospective cohort, active DA-HAI surveillance study was conducted in 3 Greek intensive care units from July 2009 to June 2010. The rates of mechanical ventilator-associated pneumonia (VAP), central catheter-associated bloodstream infection (CLABSI), and catheter-associated urinary tract infection (CAUTI) were calculated along with microbiological profile, antimicrobial resistance, and crude excess mortality.

RESULTS:

During 6004 days in intensive care, 152 of 294 patients acquired 205 DA-HAIs, an overall rate of 51.7% of patients or 34.1 DA-HAIs per 1000 days (95% CI, 29.3-38.6). The VAP rate was 20 (95% CI, 16.3-23.7) per 1000 ventilator-days, the CLABSI rate was 11.8 (95% CI: 9.2-14.8) per 1000 catheter-days, and the CAUTI rate was 4.2 (95% CI, 2.5-5.9) per 1000 catheter-days. The most frequently isolated pathogen was Acinetobacter baumannii among patients with CLABSI (37.8%) and Candida species among patients with CAUTI (66.7%). Excess mortality was 20.3% for VAP and CLABSI and 32.2% for carbapenem-resistant A baumannii CLABSI.

CONCLUSION:

High rates of DA-HAIs, device utilization, and antimicrobial resistance emphasize the need for antimicrobial stewardship, the establishment of an active surveillance program of DA-HAIs, and the implementation of evidence-based preventive strategies.

PDF

http://ajcc.aacnjournals.org/content/22/3/e12.full.pdf

February 28, 2016 at 12:35 pm

Nosocomial infections associated to invasive devices in the intensive care units of a national hospital of Lima, Peru.

Rev Peru Med Exp Salud Publica. 2013 Oct-Dec;30(4):616-20.

[Article in Spanish]

Chincha O, Cornelio E, Valverde V, Acevedo M.

Abstract

In order to describe the incidence of nosocomial infections associated to invasive devices in intensive care units (UCI) of the National Hospital Cayetano Heredia, a retrospective observational study was conducted using the data from the Office of Epidemiology and Environmental Health from 2010 to 2012.

A total number of 222 nosocomial infections were reported; the general medicine UCI reported the highest incidence of pneumonia cases associated to a mechanical ventilator in 1000 days of use of the device (28.6); infection of the blood stream associated to central venous catheter (11.9), and infection of the urinary tract associated to a catheter (8,1).

The main infectious agents isolated were Pseudomonas sp. (32.3%) in the emergency UCI, negative Staphylococcus coagulasa (36%) in the general medicine UCI and Candida sp (69.2%) in the Surgery UCI.

The rates of infections associated to invasive devices were high as in other national hospitals with limited resources and infrastructure.

PDF

http://www.scielosp.org/pdf/rpmesp/v30n4/a12v30n4.pdf

February 28, 2016 at 12:33 pm

Zika Virus Infection Among U.S. Pregnant Travelers — August 2015–February 2016

MMWR Early Release February 26, 2016 Vol. 65, Early Release

Dana Meaney-Delman, MD; Susan L. Hills, MBBS; Charnetta Williams, MD; et al.

On January 19, 2016, CDC released interim guidelines recommending pregnant women who had traveled to areas with ongoing local transmission of Zika virus and who had symptoms consistent with Zika virus disease be tested for Zika virus infection. These guidelines were updated and expanded on February 5 to offer Zika virus testing to all pregnant women with Zika virus exposure, regardless of presence of symptoms. As of February 17, 2016, nine pregnant travelers with Zika virus infection from the United States had been identified. No Zika virus–related hospitalizations or deaths were reported among pregnant women. Pregnancy outcomes among the nine confirmed cases included two early pregnancy losses, two elective terminations, and three live births (two apparently healthy infants and one infant with severe microcephaly); two pregnancies (18 weeks’ and 34 weeks’ gestation) are continuing without known complications.

PDF

http://www.cdc.gov/mmwr/volumes/65/wr/pdfs/mm6508e1er.pdf

February 26, 2016 at 9:44 pm

Transmission of Zika Virus Through Sexual Contact with Travelers to Areas of Ongoing Transmission — Continental United States, 2016

MMWR Early Release February 26, 2016 Vol. 65, Early Release

Susan L. Hills, MBBS; Kate Russell, MD; Morgan Hennessey, DVM; et al.

CDC released interim guidance for prevention of sexual transmission of Zika virus on February 5, 2016, and updated guidelines on February 26, 2016. This report provides information on six confirmed and probable cases of sexual transmission of Zika virus from male travelers to female nontravelers.

PDF

http://www.cdc.gov/mmwr/volumes/65/wr/pdfs/mm6508e2er.pdf

February 26, 2016 at 9:42 pm

Postpandemic Influenza A/H1N1pdm09 is still Causing Severe Perinatal Complications.

Mediterr J Hematol Infect Dis. 2015 Jan 1;7(1):e2015007.

Bogers H1, Bos D1, Schoenmakers S1, Duvekot JJ1.

Author information

1Erasmus MC, University Medical Centre Rotterdam, Department of Obstetrics and Gynaecology, Division of Obstetrics and Prenatal Medicine, University Medical Centre Rotterdam.

Abstract

Although influenza A/H1N1pdm09 is not causing a pandemic anymore, we recently observed two critically ill pregnant women infected by this virus.

We present these cases to illustrate the possible severe complications of an – at that moment – seasonal influenza in pregnancy.

We discuss the epidemiological differences between the pandemic and post pandemic phase and try to explain the high virulence of influenza A/H1N1pdm09 -infections in pregnancy by discussing insights in immunology during pregnancy.

We conclude that although influenza A/H1N1pdm09 is in the post pandemic phase, infection by this influenza virus still needs to be considered in pregnant women with progressive respiratory dysfunction.

PDF

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4283922/pdf/mjhid-7-1-e2015007.pdf

February 26, 2016 at 7:52 am

Candida urinary tract infections – epidemiology.

Clin Infect Dis. 2011 May;52 Suppl 6:S433-6.

Sobel JD1, Fisher JF, Kauffman CA, Newman CA.

Author information

1Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan, USA. jsobel@med.wayne.edu

Abstract

Candiduria is rarely present in healthy individuals. In contrast, it is a common finding in hospitalized patients, especially those in intensive care units (ICUs) who often have multiple predisposing factors, including diabetes mellitus, indwelling urinary catheters, and exposure to antimicrobials.

Candiduria occurs much less commonly in the community setting. In a majority of episodes in adult patients in critical care facilities candiduria represents colonization, and antifungal therapy is not required.

However, the presence of yeast in the urine can be a sign of a disseminated infection. In the critically ill newborn, candiduria often reflects disseminated candidiasis and is accompanied by obstructing fungus ball formation in the urinary tract.

In ICU patients, although candiduria is a marker for increased mortality, it is only rarely attributable to Candida urinary tract infection

PDF

http://cid.oxfordjournals.org/content/52/suppl_6/S433.full.pdf

February 26, 2016 at 7:49 am

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