Multicenter Evaluation of MRSASelect II Chromogenic Agar for Identification of Methicillin-Resistant Staphylococcus aureus from Wound and Nasal Specimens

February 6, 2016 at 8:20 am

Journal of Clinical Microbiology February 2016 V.54 N.2 P.305-311

Diana R. Hernandez, Duane W. Newton, Nathan A. Ledeboer, Blake Buchan, Carol Young, Andrew E. Clark, Jessica Connoly, and Donna M. Wolk

aGeisinger Health System, Danville, Pennsylvania, USA

bUniversity of Michigan Health System, Ann Arbor, Michigan, USA

cMedical College of Wisconsin, Milwaukee, Wisconsin, USA

dUniversity of Arizona, Tucson, Arizona, USA

Hospitals strive to reduce methicillin-resistant Staphylococcus aureus (MRSA) prevalence via active surveillance of inpatient populations.

Rapid and inexpensive screening methods are utilized when molecular methods are not operationally feasible. In this multisite clinical trial, the utility of Bio-Rad’s MRSASelect II was evaluated for MRSA identification from remnant nares and wound swabs.

The prevalence of MRSA was 11.1% (n = 1,384) from nares samples and 18.1% (n = 842) from wound samples. MRSASelect II had an overall concordance of 95.4% (confidence interval [CI] = 94.5% to 96.2%) compared to a broth-enriched reference standard.

Comparisons between results, stratified by examination times, exhibited a nonsignificant trend toward increased positivity at prolonged incubation times. Cefoxitin screening of colonies directly from MRSASelect II was 96.7% (95.8% to 97.3%) concordant compared to testing of colonies following broth enrichment.

A comparison of MRSASelect and MRSASelect II revealed no statistical differences; however, the latter exhibited earlier positivity, greater selectivity, and more intense indicator staining, which resulted in facilitated differentiation of positive results. MRSASelect II agar is a simple, rapid, and robust method to routinely screen patients for MRSA colonization without the need for additional testing.




Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Epidemiología, Health Care-Associated Infections, Infecciones nosocomiales, Metodos diagnosticos, Resistencia bacteriana.

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