Rapid Detection of Polymyxin Resistance in Enterobacteriaceae

May 21, 2016 at 10:41 am

Emerging Infectious Diseases June 2016 V.22 N.6

Patrice Nordmann, Aurélie Jayol, and Laurent Poirel

University of Fribourg, Fribourg, Switzerland

For identification of polymyxin resistance in Enterobacteriaceae, we developed a rapid test that detects glucose metabolization associated with bacterial growth in the presence of a defined concentration of colistin or polymyxin B. Formation of acid metabolites is evidenced by a color change (orange to yellow) of a pH indicator (red phenol). To evaluate the test, we used bacterial colonies of 135 isolates expressing various mechanisms of colistin resistance (intrinsic, chromosomally encoded, and plasmid-mediated MCR-1) and 65 colistin-susceptible isolates. Sensitivity and specificity were 99.3% and 95.4%, respectively, compared with the standard broth microdilution method. This new test is inexpensive, easy to perform, sensitive, specific, and can be completed in <2 hours. It could be useful in countries facing endemic spread of carbapenemase producers and for which polymyxins are last-resort drugs.

PDF

http://wwwnc.cdc.gov/eid/article/22/6/pdfs/15-1840.pdf

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Health Care-Associated Infections, Infecciones nosocomiales, Metodos diagnosticos, Resistencia bacteriana, Sepsis, Update. Tags: .

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