Archive for June 9, 2016

Brucella Septic Arthritis: Case Reports and Review of the Literature.

Case Rep Infect Dis. 2016;2016:4687840.

Elzein FE1, Sherbeeni N1.

Author information

1Division of Infectious Diseases, Department of Medicine, Prince Sultan Military Medical City (PSMMC), Riyadh 11159, Saudi Arabia.

Abstract

Brucellosis is one of the commonest zoonotic infections worldwide. The disease is endemic in Saudi Arabia, the Middle East, and the Mediterranean area. Osteoarticular involvement is a frequent manifestation of brucellosis. It tends to involve the sacroiliac joints more commonly; however, spondylitis and peripheral arthritis are increasingly reported. Brucellosis can be overlooked especially in the presence of companion bacteria. Hence, it should be suspected in all patients with septic arthritis in endemic areas or in patients visiting such areas.

PDF

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856911/pdf/CRIID2016-4687840.pdf

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June 9, 2016 at 2:57 pm

Testicular infection in brucellosis: Report of 34 cases.

J Microbiol Immunol Infect. 2016 Mar 10. pii: S1684-1182

Bosilkovski M1, Kamiloski V2, Miskova S3, Balalovski D4, Kotevska V5, Petrovski M2.

Author information

1Medical Faculty “Ss Cyril and Methodius University”, Skopje, Macedonia. Electronic address: milebos@yahoo.com

2Medical Faculty “Ss Cyril and Methodius University”, Skopje, Macedonia.

3Department for Infectious Diseases, Medical Center, Veles, Macedonia.

4Department for Infectious Diseases, Medical Center, Bitola, Macedonia.

5Medical Faculty “Ss Cyril and Methodius University”, Skopje, Macedonia; Institute for Clinical Microbiology, Skopje, Macedonia.

Abstract

BACKGROUND/PURPOSE:

To present clinical and laboratory features, treatment options, and outcome in patients with brucellar testicular infection and to compare them with analogous in brucellar patients without testicular involvement.

METHODS:

Thirty four brucellar patients with testicular infection treated in two general hospitals in the Republic of Macedonia, during the period 1998-2009, were retrospectively analyzed. Their clinical and laboratory characteristics were compared with analogous in 364 male brucellar patients without testicular infection, who were treated at the same hospitals during the same time period.

RESULTS:

Brucellar testicular infection was evident in 34 (8.5%) out of 398 male patients with brucellosis. The median age of the patients was 46.5 years. In all patients testicular involvement was presented as an acute form with a median duration of 5 days (range, 2-14 days) prior to diagnosis. Twenty-three of the patients had at least one other simultaneous focal infection. After starting with the treatment testicular infection lasted a median 10 days, range 7-21 days. Brucellar patients with testicular infection when compared with other brucellar patients more frequently manifested fever (97% vs. 61%), concomitant spondylitis (32% vs. 16%), and urinary system involvement (12% vs. 2%). Also, the relapse rate in patients with testicular involvement was significantly higher (24% vs. 9%).

CONCLUSION:

In endemic regions brucellosis should be taken into consideration in any patient with testicular infection. Brucellar testicular involvement is usually characterized with a severe acute clinical presentation and a high percentage of relapses which entails the need of timely recognition and proper treatment duration of at least 60 days.

PDF

http://www.e-jmii.com/article/S1684-1182(16)30004-4/pdf

June 9, 2016 at 2:56 pm

Comparison of the tests polymerase chain reaction, serology, and blood culture with respect to sensitivity and specificity for detection of Brucella spp in human samples.

 Gac Med Mex. 2015 Sep-Oct;151(5):620-7.

Article in Spanish

Álvarez-Ojeda MG1, Saldaña-Fuentes C2, Ballesteros-Elizondo MR3, Martínez-Vázquez IO2, López-Merino A4, Briones Lara E5, Morales-Loredo A6.

Author information

1Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Centro de Investigación Regional del Noreste, Río Bravo, Tamps., México.

2Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, San Nicolás de los Garza, N.L., México.

3Servicios de Salud de Nuevo León, Laboratorio Estatal de Salud Pública de Nuevo León, Guadalupe, N.L., México.

4Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., México.

5Instituto Mexicano del Seguro Social, Hospital de Especialidades Gineco-Obstétricas “Dr. Ignacio Morones Prieto”, Monterrey, N.L., México.

6Universidad Autónoma de Nuevo León (UANL), Centro de Desarrollo de Agronegocios, General Escobedo, N.L., México.

Abstract

OBJECTIVE:

The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction for detection of Brucella spp in human blood samples compared with the serological tests and blood culture.

MATERIAL AND METHODS:

In 2005, a total of 92 people were sampled from the towns of Anahuac and Sabinas Hidalgo, Nuevo Leon, where an outbreak of human cases had taken place in the same year as this study. The sera collected were analyzed by serological tests according to the NOM 022-SS2-1994. DNA was obtained using CTAB extraction method and it was used to amplify a fragment of 223 bp of the coding sequence for a protein of 31 kDa present in all Brucella species.

RESULTS:

The polymerase chain reaction test detected 23 positive samples. The sensitivity and specificity compared with RB was 44.68 and 95.56%, respectively. Compared with mouse antibody production, it was 51.61 and 88.52%, and 2-mercaptoethanol was 53.57 and 87.50%. When isolation (positives cultures) was compared with polymerase chain reaction, we obtained 100.0% sensitivity and 80.23% specificity, taking into account people with positive and negative serology.

CONCLUSIONS:

The polymerase chain reaction test can be an alternative tool to bacterial culture in human brucellosis diagnosis

PDF

http://www.anmm.org.mx/GMM/2015/n5_english/2331AX155_151_2015_UK5_579-585.pdf

June 9, 2016 at 2:54 pm

Comparison of Test Results for Zika Virus RNA in Urine, Serum, and Saliva Specimens from Persons with Travel-Associated Zika Virus Disease – Florida, 2016.

MMWR Morb Mortal Wkly Rep. 2016 May 13;65(18):475-8.

Bingham AM1, Cone M, Mock V, Heberlein-Larson L, Stanek D, Blackmore C, Likos A.

1Florida Department of Health.

Abstract

In May 2015, Zika virus was reported to be circulating in Brazil. This was the first identified introduction of the virus in the Region of the Americas. Since that time, Zika virus has rapidly spread throughout the region.

As of April 20, 2016, the Florida Department of Health Bureau of Public Health Laboratories (BPHL) has tested specimens from 913 persons who met state criteria for Zika virus testing.

Among these 913 persons, 91 met confirmed or probable Zika virus disease case criteria and all cases were travel-associated (1). On the basis of previous small case studies reporting real time reverse-transcription polymerase chain reaction (RT-PCR) detection of Zika virus RNA in urine, saliva, and semen (2-6), the Florida Department of Health collected multiple specimen types from persons with suspected Zika virus disease.

Test results were evaluated by specimen type and number of days after symptom onset to determine the most sensitive and efficient testing algorithm for acute Zika virus disease. Urine specimens were collected from 70 patients with suspected Zika virus disease from zero to 20 days after symptom onset.

Of these, 65 (93%) tested positive for Zika virus RNA by RT-PCR. Results for 95% (52/55) of urine specimens collected from persons within 5 days of symptom onset tested positive by RT-PCR; only 56% (31/55) of serum specimens collected on the same date tested positive by RT-PCR.

Results for 82% (9/11) of urine specimens collected >5 days after symptom onset tested positive by RT-PCR; none of the RT-PCR tests for serum specimens were positive. No cases had results that were exclusively positive by RT-PCR testing of saliva. BPHL testing results suggest urine might be the preferred specimen type to identify acute Zika virus disease.

PDF

http://www.cdc.gov/mmwr/volumes/65/wr/pdfs/mm6518e2.pdf

June 9, 2016 at 2:15 pm


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