Microbiological laboratory diagnostics of neglected zoonotic diseases (NZDs).

June 12, 2016 at 7:47 pm

Acta Trop. 2015 Sep 21. pii: S0001-706X(15)30101-7.

Schwarz NG1, Loderstaedt U2, Hahn A3, Hinz R4, Zautner AE5, Eibach D6, Fischer M7, Hagen RM8, Frickmann H9.

Author information

1Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: schwarznorbert@bnitm.de

2Institute for Clinical Chemistry, University Medical Centre, D-37075 Göttingen, Germany. Electronic address: ulrike.loderstaedt@med.uni-goettingen.de

3Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: hahn@bnitm.de

4Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: hinz@bnitm.de

5Institute for Clinical Chemistry, University Medical Centre, D-37075 Göttingen, Germany; Institute for Medical Microbiology, University Medical Centre, D-37075 Göttingen, Germany. Electronic address: azautne@gwdg.de

6Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: eibach@bnitm.de

7Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: marcellusfischer@bundeswehr.org

8Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard Nocht street 74, D-20359 Hamburg, Germany. Electronic address: hagen@bnitm.de

9Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Bernhard Nocht street 74, D-20359 Hamburg, Germany; Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, D-18057 Rostock, Germany. Electronic address: Frickmann@bni-hamburg.de

Abstract

This review reports on laboratory diagnostic approaches for selected, highly pathogenic neglected zoonotic diseases, i.e. anthrax, bovine tuberculosis, brucellosis, echinococcosis, leishmaniasis, rabies, Taenia solium-associated diseases (neuro-/cysticercosis & taeniasis) and trypanosomiasis. Diagnostic options, including microscopy, culture, matrix-assisted laser-desorption-ionisation time-of-flight mass spectrometry, molecular approaches and serology are introduced.

These procedures are critically discussed regarding their diagnostic reliability and state of evaluation. For rare diseases reliable evaluation data are scarce due to the rarity of samples. If bio-safety level 3 is required for cultural growth, but such high standards of laboratory infrastructure are not available, serological and molecular approaches from inactivated sample material might be alternatives.

Multiple subsequent testing using various test platforms in a stepwise approach may improve sensitivity and specificity. Cheap and easy to use tests, usually called “rapid diagnostic tests” (RDTs) may impact disease control measures, but should not preclude developing countries from state of the art diagnostics.

PDF

http://ac.els-cdn.com/S0001706X15301017/1-s2.0-S0001706X15301017-main.pdf?_tid=c5f80970-30eb-11e6-a7a1-00000aacb35d&acdnat=1465770167_1e320f028d784d29a36812f4b0d42c69

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Epidemiología, F.O.D, Metodos diagnosticos, REVIEWS, Sepsis, Update, Zoonosis. Tags: .

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