Application of Mass Spectrometry Technology to Early Diagnosis of Invasive Fungal Infections

October 26, 2016 at 8:05 am

Journal of Clinical Microbiology November 2016 V.54 N.11 P.2786-2797

Alexandre Mery, Boualem Sendid, Nadine François, Marjorie Cornu, Julien Poissy, Yann Guerardel, and Daniel Poulain

aSATT Nord-de-France, Lille, France

bUniversité Lille, CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Lille, France

cCHU Lille, Service de Parasitologie-Mycologie, Lille, France

dUniversité Lille, U995-LIRIC-Lille Inflammation Research International Centre, Lille, France

eINSERM, U995-Team 2, Lille, France

fCHU Lille, Pôle de Réanimation, Lille, France

gCHU Lille, Délégation à la Recherche Clinique et à l’Innovation, Lille, France

We recently developed a mass spectrometry (MS) procedure based on the detection of a serum disaccharide (MS-DS) in patients with invasive candidiasis (IC).

Here, we compare the performance of MS-DS for the diagnosis of IC, invasive aspergillosis (IA), and mucormycosis (MM) with those of commercially available antigen detection tests.

This retrospective study included 48 patients (23 IC patients [74 serum samples], 15 IA patients [40 serum samples], and 10 MM patients [15 serum samples]) and 49 appropriate controls (102 serum samples). MS-DS, mannan (Mnn), galactomannan (GM), and (1,3)-β-D-glucan (BDG) were detected by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS, Platelia, and Fungitell assays, respectively.

For IC, the sensitivity and specificity of the MS-DS index, BDG detection, and Mnn detection were 62% and 84%, 82% and 60%, and 33% and 94% per serum sample and 83% and 69%, 96% and 31%, and 39% and 86% per patient, respectively.

For IA, the corresponding values in comparison to BDG and GM detection were 83% and 81%, 62% and 95%, and 62% and 100% per serum sample and 93% and 76%, 87% and 90%, and 93% and 100% per patient, respectively. Nine of the 10 MM patients had a positive MS-DS result. MS-DS gave an early diagnosis in IC (73% positivity before blood culture), IA (positive before GM detection in six patients), and MM (positivity mainly preceded the date of diagnosis) patients.

For IC, persisting MS-DS was associated with a poor prognosis. The different biomarkers were rarely detected simultaneously, suggesting different kinetics of release and clearance. For IA, MS-DS provided better complementation to GM monitoring than BDG monitoring. MS-DS detects panfungal molecules circulating during invasive fungal infections.

The performance of MS-DS compared favorably with those of biological tests currently recommended for monitoring at-risk patients. Further validation of this test in multicenter studies is required.



Entry filed under: Biología Molecular, Epidemiología, Infecciones micoticas, Metodos diagnosticos, Sepsis, Update.

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