Analysis of linezolid and tigecycline as candidates for local prophylaxis via antibiotic-loaded bone cement

January 24, 2017 at 8:07 am

Journal of Antimicrobial Chemotherapy February 2017 V.72 N.2 P.410–416

EDITOR’S CHOICE

Nichol; T. J. Smith; R. Townsend; I. Stockley; R. Akid

Objectives

To assess the Gram-positive-specific antibiotic linezolid and the broad-spectrum antibiotic tigecycline for use in local antibiotic delivery via antibiotic-loaded bone cement.

Methods

Linezolid and tigecycline were added to Biomet bone cement at varying concentrations. Antibiotic elution over 1 week was quantified by HPLC-MS. The effect of wear on elution over 51 h was determined using a modified TE-66 wear tester. Eluted antibiotics were used to determine the MICs for a panel of clinically relevant bacteria. The impact strength of antibiotic-loaded samples was determined using a Charpy-type impact testing apparatus. Cytotoxicity of eluted antibiotics against MG-63 cells was evaluated using an MTT assay.

Results

Linezolid and tigecycline eluted from bone cement to clinically relevant levels within 1 h and retained activity over 1 week. Mechanical wear significantly reduced elution of tigecycline, but had little effect on elution of linezolid. Linezolid showed low cytotoxicity towards MG-63 cells with ≤300 mg/mL resulting in >50% cell activity. Cytotoxicity of tigecycline was higher, with an IC50 of 5–10 mg/L.

Conclusions

Linezolid and tigecycline retain activity after elution from bone cement. The concentration of tigecycline may need to be carefully controlled due to cytotoxicity. The effect of wear on bone cement may need to be considered if tigecycline is to be used for local delivery. Up to 10% linezolid can be added without affecting the impact strength of the bone cement. These results are promising indications for future investigation of these antibiotics for use in local antibiotic delivery strategies.

FULL TEXT

https://academic.oup.com/jac/article/72/2/410/2290944/Analysis-of-linezolid-and-tigecycline-as

PDF

http://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jac/72/2/10.1093_jac_dkw410/3/dkw410.pdf?Expires=1485599246&Signature=KwUBK1~CIeGnAogtq1WNjsOKzrTY-QCiw9Q79t2l-CX6rqIV5Bu0xhqwV5VmwVgL9JFDusD1Tl82Il1Q3Qrc21IJjKbOfcgpdIGIVUPlWJ0tWkJ5k1aQ3oXYoiwpIUaYoUS7Jr7Lt7mlYfc~e44UslkOk6U0LCih311wG~EYHXrm2BR-MD-hL4KMIYwcb-hxxAeubHhnoJxcktSrkI1wHLEMvz9OmqKNLDkPj7DEjG2ghZ-2F3tCCka98J4M3DmuEPlhtrwSjPYYCJ9V~0-CWkSEb-eYqa5wX9LeG4BOV-eyBf861aSp2tw5ZhqHuMkeHFxdRc7Vv9iFsEk590EWmQ__&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q

Advertisements

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Infecciones osteo-articulares-musculares, Infecciones relacionadas a prótesis, Metodos diagnosticos, REPORTS, Resistencia bacteriana, Sepsis, Update.

Linezolid: a promising option in the treatment of Gram-positives Review – Role of cephalosporins in the era of Clostridium difficile infection


Calendar

January 2017
M T W T F S S
« Dec   Feb »
 1
2345678
9101112131415
16171819202122
23242526272829
3031  

Most Recent Posts


%d bloggers like this: