Archive for May 4, 2017

Complex Routes of Nosocomial Vancomycin-Resistant Enterococcus faecium Transmission Revealed by Genome Sequencing.

Clin Infect Dis. 2017 Apr 1;64(7):886-893.

Raven KE1, Gouliouris T1,2,3, Brodrick H1, Coll F4, Brown NM2,3, Reynolds R5,6, Reuter S1, Török ME1,2,3, Parkhill J7, Peacock SJ1,3,4,7.

Author information

1 Department of Medicine, University of Cambridge.

2 Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke’s Hospital, and.

3 Cambridge University Hospitals NHS Foundation Trust, Cambridge.

4 London School of Hygiene and Tropical Medicine.

5 British Society for Antimicrobial Chemotherapy, Birmingham.

6 North Bristol NHS Trust, Southmead Hospital, Bristol; and.

7 Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.



Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of nosocomial infection. Here, we describe the utility of whole-genome sequencing in defining nosocomial VREfm transmission.


A retrospective study at a single hospital in the United Kingdom identified 342 patients with E. faecium bloodstream infection over 7 years. Of these, 293 patients had a stored isolate and formed the basis for the study. The first stored isolate from each case was sequenced (200 VREfm [197 vanA, 2 vanB, and 1 isolate containing both vanA and vanB], 93 vancomycin-susceptible E. faecium) and epidemiological data were collected. Genomes were also available for E. faecium associated with bloodstream infections in 15 patients in neighboring hospitals, and 456 patients across the United Kingdom and Ireland.


The majority of infections in the 293 patients were hospital-acquired (n = 249) or healthcare-associated (n = 42). Phylogenetic analysis showed that 291 of 293 isolates resided in a hospital-associated clade that contained numerous discrete clusters of closely related isolates, indicative of multiple introductions into the hospital followed by clonal expansion associated with transmission. Fine-scale analysis of 6 exemplar phylogenetic clusters containing isolates from 93 patients (32%) identified complex transmission routes that spanned numerous wards and years, extending beyond the detection of conventional infection control. These contained both vancomycin-resistant and -susceptible isolates. We also identified closely related isolates from patients at Cambridge University Hospitals NHS Foundation Trust and regional and national hospitals, suggesting interhospital transmission.


These findings provide important insights for infection control practice and signpost areas for interventions. We conclude that sequencing represents a powerful tool for the enhanced surveillance and control of nosocomial E. faecium transmission and infection




May 4, 2017 at 8:51 am

Complications of Mumps During a University Outbreak Among Students Who Had Received 2 Doses of Measles-Mumps-Rubella Vaccine – Iowa, July 2015-May 2016.

MMWR Morb Mortal Wkly Rep. Apr 14, 2017 V.66 N.14 P.390-391.

Notes from the Field:

Donahue M, Schneider A, Ukegbu U, Shah M, Riley J, Weigel A, James L, Wittich K, Quinlisk P, Cardemil C.


May 4, 2017 at 8:50 am


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