The Diagnostic Utility of Induced Sputum Microscopy and Culture in Childhood Pneumonia

June 3, 2017 at 2:28 pm

Clinical Infectious Diseases June 15, 2017 V.64 Suppl.3

David R. Murdoch; Susan C. Morpeth; Laura L. Hammitt; Amanda J. Driscoll; Nora L. Watson …

Background.

Sputum microscopy and culture are commonly used for diagnosing the cause of pneumonia in adults but are rarely performed in children due to difficulties in obtaining specimens. Induced sputum is occasionally used to investigate lower respiratory infections in children but has not been widely used in pneumonia etiology studies.

Methods.

We evaluated the diagnostic utility of induced sputum microscopy and culture in patients enrolled in the Pneumonia Etiology Research for Child Health (PERCH) study, a large study of community-acquired pneumonia in children aged 1–59 months. Comparisons were made between induced sputum samples from hospitalized children with radiographically confirmed pneumonia and children categorized as nonpneumonia (due to the absence of prespecified clinical and laboratory signs and absence of infiltrate on chest radiograph).

Results.

One induced sputum sample was available for analysis from 3772 (89.1%) of 4232 suspected pneumonia cases enrolled in PERCH. Of these, sputum from 2608 (69.1%) met the quality criterion of <10 squamous epithelial cells per low-power field, and 1162 (44.6%) had radiographic pneumonia. Induced sputum microscopy and culture results were not associated with radiographic pneumonia, regardless of prior antibiotic use, stratification by specific bacteria, or interpretative criteria used.

Conclusions.

The findings of this study do not support the culture of induced sputum specimens as a diagnostic tool for pneumonia in young children as part of routine clinical practice.

PDF

https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/cid/64/suppl_3/10.1093_cid_cix090/5/cix090.pdf?Expires=1496600154&Signature=b54n~53ADNgzxYtJ1QQKlQ0bZtUrjqhzvRwe1kpsMh0xPIZHn4iOzD-wwGVMH12apmysb1JeCZu6DblvT0KnYrg8Yc8ODa9ZU3gu5qVKYbAphhdlA8IfRNHBniNDmQS9ok4~kjWrj7vI3eJoVVw96TXlvUDyXU1dBosYVWTQbyCkdAPnHTuGSVyvPJQDQg0NrrSFDN5vJmqXgqn92-2SJVttIHL~CdKsz-DMaeNKoaYUzRsdcsfcU0iuks30jblHeb02wk~dW1LrTtaVJ44PK4J8nbUlQs5XRBkFBKGtY8IublgVkDv1AkV6dCl5-iWLyEsXFi36NJ3DPbZ5ohL3WA__&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q

Advertisements

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Epidemiología, Infecciones respiratorias, Infecciones virales, Inmunizaciones, Metodos diagnosticos, Sepsis, Update.

Microscopic Analysis and Quality Assessment of Induced Sputum From Children With Pneumonia in the PERCH Study Limited Utility of Polymerase Chain Reaction in Induced Sputum Specimens for Determining the Causes of Childhood Pneumonia in Resource-Poor Settings: Findings From the Pneumonia Etiology Research for Child Health (PERCH) Study


Calendar

June 2017
M T W T F S S
« May   Jul »
 1234
567891011
12131415161718
19202122232425
2627282930  

Most Recent Posts


%d bloggers like this: