Staphylococcus coagulasa-negativa clínicamente significativos. Especies más frecuentes y factores de virulencia

July 6, 2017 at 9:17 pm

Rev Chilena Infectol 2013; 30 (5): 480-488

Norma Fariña, Letizia Carpinelli, Margarita Samudio, Rosa Guillén, Florentina Laspina, Ramona Sanabria, Sonia Abente, Ladis Rodas, Pedro González y Herminia M. de Kaspar

Instituto de Investigaciones en Ciencias de la Salud Asunción, Paraguay. (NF, LC, MS, RG, FL, RS, SA, HM). Laboratorio San Roque. Asunción, Paraguay (LR, PG).

Background

Coagulase-negative staphylococci have emerged as responsible for a large number of infections. However, it is often difficult to assess its pathogenic role or to discard it as a contaminant.

Aim

The goal of this study was to identify clinically significant coagulase-negative staphylococci to the species level and their virulence factors. Isolates came from patients consulting at the San Roque Laboratory from 2009 to 2011.

Material and Methods

Species identification was performed by De Paulis et al simplified method. Production of biofilm, hemolysins, lipases, lecithinases and DNase were determined by conventional methods; methicillin-resistance by diffusion method and mecA and Panton-Valentine genes, by multiplex PCR.

Results

Out of 64 isolates, 40.6% were S. epidermidis; 20.3%, S. haemolyticus, and 15.6%, S. lugdunensis. Biofilm production was detected in 73.1% of S. epidermidis, 53.8% of S. haemolyticus and 40% of S. lugdunensis. mecA gene was identified in 69.2% of S. epidermidis, 92.3% of S. haemolyticus and none of S. lugdunensis. 83% of mecA (+) S. epidermidis isolates were biofilm producers as compared to 50% of the mecA (-).

Conclusion

The frequency of S. lugdunensis, the most virulent coagulase-negative staphylococci species, was relatively high. The main virulence factor in S. epidermidis was biofilm production, being higher in those resistant to methicillin.

PDF

http://www.scielo.cl/pdf/rci/v30n5/art03.pdf

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Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Biología Molecular, Epidemiología, Metodos diagnosticos, REPORTS, Resistencia bacteriana, Sepsis, Update.

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