First case of New Delhi metallo-β-lactamase in Klebsiella pneumoniae from Ecuador: An update for South America

February 10, 2018 at 9:09 am

International Journal of Infectious Diseases December 2017 V.65 N. P.119–121

Daniel Romero-Alvarez, Jorge Reyes, Viviana Quezada, Carolina Satán, Nelson Cevallos, Sofía Barrera, Gabriel Trueba, Luis E. Escobar, José E. Villacís

Highlights

  • The New Delhi metallo-β-lactamase (NDM) resistance plasmid has autochthonous circulation in Ecuador.
  • A Klebsiella pneumoniae ST147 harboring the NDM-1 gene in an IncA/C plasmid is described for the first time in Quito, Ecuador.
  • The circulation of NDM in South America has been addressed mainly by Brazil and Colombia.

Objectives

To describe a clinical case of Klebsiella pneumoniae harboring a New Delhi metallo-β-lactamase (NDM) plasmid in Ecuador and to present a map of reports of NDM isolates in South America.

Methods

The modified Hodge test, carbapenem inactivation method, imipenem–EDTA disk method (synergy), and Rapidec Carba NP test were used to identify antibiotic resistance mechanisms. The presence of resistance genes was explored with a conjugation assay, and molecular confirmation of NDM was performed by PCR and DNA sequencing. Plasmid characterization was conducted by PCR-based replicon typing. A literature review was performed in Google Scholar and PubMed to identify reports from South America.

Results

An HIV-infected patient, who had never traveled abroad, developed a bloodstream infection caused by K. pneumoniae ST147 harboring the NDM-1 resistance gene in a plasmid from the IncA/C group. Local circulation of NDM has also been described in other South American countries, in particular in Colombia and Brazil, although published scientific records were not found for other countries.

Conclusions

This report presents the first evidence of autochthonous circulation of the NDM-1 resistance gene harbored by an IncA/C plasmid isolated from a K. pneumoniae ST147 in Ecuador. Efforts should be implemented to monitor and characterize the spatial and temporal distribution of NDM in Ecuador and other countries of South America.

abstract

http://www.ijidonline.com/article/S1201-9712(17)30268-0/fulltext

PDF

http://www.ijidonline.com/article/S1201-9712(17)30268-0/pdf

Advertisements

Entry filed under: Antimicrobianos, Bacterias, Bacteriemias, Biología Molecular, Epidemiología, Metodos diagnosticos, REPORTS, Resistencia bacteriana, Sepsis, Update.

Adenovirus Type 4 Respiratory Infections among Civilian Adults, Northeastern United States, 2011–2015 Seroprevalence and risk factors of Hepatitis E infection in Jordan’s population: First report


Calendar

February 2018
M T W T F S S
« Jan   Mar »
 1234
567891011
12131415161718
19202122232425
262728  

Most Recent Posts


%d bloggers like this: