Enterotoxigenic Escherichia coli blood group A interactions intensify diarrheal severity

May 21, 2018 at 8:38 am

The Journal of Clinical Investigation 2018

Pardeep Kumar, Mark Donowitz, James M. Fleckenstein

Enterotoxigenic Escherichia coli (ETEC) infections are highly prevalent in developing countries where clinical presentations range from asymptomatic colonization to severe cholera-like illness. The molecular basis for these varied presentations, that may involve strain-specific virulence features as well as host factors, have not been elucidated. We demonstrated that when challenged with ETEC strain H10407, originally isolated from a case of cholera-like illness, blood group A human volunteers developed severe diarrhea more frequently than individuals from other blood groups. Interestingly, a diverse population of ETEC strains, including H10407, secrete the EtpA adhesin molecule. As many bacterial adhesins also agglutinate red blood cells, we combined the use of glycan arrays, biolayer inferometry, and non-canonical amino acid labeling with hemagglutination studies to demonstrate that EtpA is a dominant ETEC blood group A specific lectin/hemagglutinin. Importantly, we have also shown that EtpA interacts specifically with glycans expressed on intestinal epithelial cells from blood group A individuals, and that EtpA-mediated bacterial-host interactions accelerate bacterial adhesion and effective delivery of both the heat-labile and heat-stable toxins of ETEC. Collectively, these data provide additional insight into the complex molecular basis of severe ETEC diarrheal illness that may inform rational design of vaccines to protect those at highest risk.

abstract

https://www.jci.org/articles/view/97659/pdf

PDF (CLIC en DOWNLOAD)

https://dm5migu4zj3pb.cloudfront.net/manuscripts/97000/97659/cache/97659.1-20180507184523-covered-253bed37ca4c1ab43d105aefdf7b5536.pdf

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Entry filed under: Bacterias, Epidemiología, Infecciones gastrointestinales, Metodos diagnosticos, REPORTS, Update.

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